Journal:

Article Title: Expression of prostanoid receptors in human ductus arteriosus

doi: 10.1038/sj.bjp.0705092

Figure Lengend Snippet: Primer design for PCR amplification of prostanoid receptors and β-actin

Article Snippet: Abundance of receptor mRNA was expressed relative to β-actin mRNA. table ft1 table-wrap mode="anchored" t5 caption a7 Primer design for PCR amplification of prostanoid receptors and β -actin Open in a separate window

Techniques: Amplification

Journal:

Article Title: Expression of prostanoid receptors in human ductus arteriosus

doi: 10.1038/sj.bjp.0705092

Figure Lengend Snippet: Expression of prostanoid receptor mRNA in human DA. RNA was isolated from excised tissue, reverse-transcribed by oligo(dT) and PCR amplification for all tissue samples was repeated three times. A fragment of β-actin was amplified as internal control. A representative expression pattern of one tissue sample is shown, base pair markers (bp) are indicated on both sides.

Article Snippet: Abundance of receptor mRNA was expressed relative to β-actin mRNA. table ft1 table-wrap mode="anchored" t5 caption a7 Primer design for PCR amplification of prostanoid receptors and β -actin Open in a separate window

Techniques: Expressing, Isolation, Reverse Transcription, Amplification, Control

Journal:

Article Title: Expression of prostanoid receptors in human ductus arteriosus

doi: 10.1038/sj.bjp.0705092

Figure Lengend Snippet: Expression of the different prostanoid receptors relative to the expression of β-actin. RNA was isolated from excised tissue, reverse-transcribed by oligo(dT) and PCR amplification was performed for the indicated prostanoid receptors. Intensity of DNA bands is presented as percentage of amplification of β-actin fragment.

Article Snippet: Abundance of receptor mRNA was expressed relative to β-actin mRNA. table ft1 table-wrap mode="anchored" t5 caption a7 Primer design for PCR amplification of prostanoid receptors and β -actin Open in a separate window

Techniques: Expressing, Isolation, Reverse Transcription, Amplification

Journal: BMC Genomics

Article Title: Natural allelic variations of Saccharomyces cerevisiae impact stuck fermentation due to the combined effect of ethanol and temperature; a QTL-mapping study

doi: 10.1186/s12864-019-5959-8

Figure Lengend Snippet: Genetic material and experimental design. a summarizes the construction of the genetic material used in this study. The H4 hybrid was obtained by a backcross program using the parental strains G-4A (G) and B-1A (B). The F1-hybrid was sporulated and the resulting segregants were phenotyped for their fermentation performance at 28 °C. The segregant leaving the smallest quantity of residual sugars was cross with the strain G-4A. This procedure was recurrently done four time in order to get the hybrid H4 that constitutes the starting point of this present study . Phenotypic comparison of the hybrid H4 and G illustrates that fermentation efficiency of H4 was specifically improved at 28 °C as reported by Marullo et al. . b describes the strategy used for mapping the chromosomal portion of the strain B-1A present in the hybrid H4. In order to narrow the most relevant regions, a selective genotyping approach was applied. Seventy-seven H4-segregants were fermented and the seven best ones were genotyped by combining Tiling Microarray (Affymetrix®) and whole genome sequencing. c describes the QTL mapping strategy applied that was carried out by developing qPCR-based markers (KASP™ technology) in order to achieve a linkage analysis using up to 160 segregants. Candidates genes identified were then validated by reciprocal hemizygosity assay (RHA)

Article Snippet: Primers design and genotyping were performed by LGC genomics (Hertz, UK).

Techniques: Comparison, Microarray, Sequencing

Journal: BMC Genomics

Article Title: Natural allelic variations of Saccharomyces cerevisiae impact stuck fermentation due to the combined effect of ethanol and temperature; a QTL-mapping study

doi: 10.1186/s12864-019-5959-8

Figure Lengend Snippet: QTL regions narrowed by selective genotyping. a . Distribution of the residual sugars found at the end of the alcoholic fermentation for the 77 H4-segregants and for the parental strains. The average values of parental strains and H4-hybrid were indicated by green (B-1A), red (G-4A) and black squares (H4-hybrid). The segregants values are the means of experimental duplicates, the seven best progenies (black dots) were selected for narrowing the QTL regions. b , Physical map of all the B-1A and G-4A specific markers inherited in the seven H4 progenies. Each thick is one of the 1204 bi-allelic markers selected. The B and G alleles are shown in green and red, respectively. The green dots are the SNP that were found in more than four segregants defining 12 chromosomal regions

Article Snippet: Primers design and genotyping were performed by LGC genomics (Hertz, UK).

Techniques: